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Gopinath, P.
- Detection of FNBA Gene Encoding for Fibronectin Binding Protein among Clinical Isolates of Staphylococcus aureus
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Authors
Affiliations
1 Saveetha Dental College, Saveetha University, Chennai, IN
2 Department of Microbiology, Saveetha Dental College, Saveetha University, Chennai, IN
1 Saveetha Dental College, Saveetha University, Chennai, IN
2 Department of Microbiology, Saveetha Dental College, Saveetha University, Chennai, IN
Source
Research Journal of Pharmacy and Technology, Vol 10, No 2 (2017), Pagination: 378-380Abstract
Staphylococcus aureus is a major human pathogen associated with nosocomial respiratory tract infections in health care settings, particularly in critical care units. Pathogenesis of S. aureus is contributed to the expression of cell surface protein receptors for Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs) that interact specifically with proteins present in the host plasma and extracellular matrix.[1] Fibronectin-binding proteins (FnBPs) A and B are two of the multifunctional MSCRAMMs of S. aureus, which recognize fibronectin, fibrinogen, and elastin. In this study we have taken 20 clinical isolates of S. aureus which were subjected to antibiotic sensitivity pattern followed by the detection of fnbA gene by PCR. We have observed total resistance to penicillin followed by other group of drugs. 10% of our strains were found to have this gene. This indicates that the presence of this gene has the direct correlation with respiratory tract infections.Keywords
Staphylococcus aureus, Fibronectin Binding Protein, PCR, FnbA Gene.- Detection of Esp Gene for the Presence of Extracellular Surface Protein among Clinical Isolates of Enterococcus Species
Abstract Views :188 |
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Authors
Affiliations
1 Saveetha Dental College, Saveetha University, Chennai, IN
2 Department of Microbiology, Saveetha Dental College, Saveetha University, Chennai, IN
1 Saveetha Dental College, Saveetha University, Chennai, IN
2 Department of Microbiology, Saveetha Dental College, Saveetha University, Chennai, IN
Source
Research Journal of Pharmacy and Technology, Vol 10, No 5 (2017), Pagination: 1370-1372Abstract
Esp gene is a high molecular weight surface protein whose frequency is significantly increased among infection derived Enterococcus faecalis isolates. Esp gene is an Enterococcal surface protein gene which is present in the opportunistic pathogens Enterococcus faecalis and Enterococcus faecium. It has been proposed that esp gene can be used as a marker of human pollution in environmental waters. This gene has been shown to be a part of a 150-in putative pathogenicity island. A sum of 20 isolates of Enterococci were assessed for the presence of esp gene that encodes for extracellular surface protein. We have observed 5% positivity in our study. This indicates that, the pathogenicity of urinary tract infections by these pathogens might be crucially played by this gene encoding for extra cellular surface protein.Keywords
Enterococcus faecalis, Esp Gene, PCR.- Bacteriophage in Human Body A-Systematic Review
Abstract Views :152 |
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Authors
Affiliations
1 Saveetha Dental College, Chennai, IN
2 Department of Microbiology, Saveetha Dental College, Chennai, IN
1 Saveetha Dental College, Chennai, IN
2 Department of Microbiology, Saveetha Dental College, Chennai, IN
Source
Research Journal of Pharmacy and Technology, Vol 10, No 4 (2017), Pagination: 1204-1208Abstract
Bacteriophage has been a known virus from many years and one of the most abundant viruses present. Preying only on bacteria, they don't harm human body. Since many years bacteriophage studies are being carried out. Recent studies shows that bacteriophage are proving to be beneficial to human body. They act as immune barriers with mucus in human body. Also these viruses are playing an important role in vaccines. One of the main recent advancement shows Bacteriophage Therapy which includes dentistry also. This review throws a light on importance of these bacteriophages in science.Keywords
Bacteriophages, Phage Therapy, Periodontitis.- Detection of PVL gene for the presence of Leukocidin among Clinical Isolates of Staphylococcus aureus from Tertiary Care Hospital
Abstract Views :151 |
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Authors
R. Gowtham
1,
P. Gopinath
2
Affiliations
1 BDS, Saveetha Dental College, Chennai, IN
2 Department of Microbiology, Saveetha Dental College, Chennai, Tamil Nadu, IN
1 BDS, Saveetha Dental College, Chennai, IN
2 Department of Microbiology, Saveetha Dental College, Chennai, Tamil Nadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 1 (2019), Pagination: 172-174Abstract
PVL and γ -haemolysin are considered to be members of a toxin family known as synergohymenotropic toxins, as they act on cell membranes by the synergy of two proteins that form a pore. Only 2% of S. aureus isolates produce PVL, while γ -haemolysin is produced by more than 99% of S. aureus isolates. PVL is the most leukocytolytic toxin in the family, however it does not exhibits no haemolytic activity on human erythrocytes. A sum of 20 clinical isolates of S. aureus were subjected to antibiotic sensitivity pattern followed by the detection of pvl gene by PCR. We have observed increased resistance to most of the routinely used antibiotics and 10% of our isolates found to have pvl gene. As this gene is directly associated with skin and soft tissue infections by S. aureus, our two isolates may even cause such infections, although none of these strains were not obtained from cutaneous lesions.Keywords
Staphylcoccus Aures, Pvl Gene, PCR.References
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- Criebier B, Pre´vost G, Couppie´ P, Finck-Barbancon V, Grosshans E, Pie´mont Y. Staphylococcus aureus leukocidin: a new virulence factor in cutaneous infections? An epidemiological and experimental study. Dermatology 1992:185, 175–180.
- Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Disk Tests, Approved Standards; Doucement M2-A9, 9th ed., Vol 26. Wayne, PA: CLSI, 2015
- Terry Alli OA, Ogbolu DO, Mustapha JO, Akinbami R, Ajayi AO. The non-association of Panton-Valentine leukocidin and mecA genes in the genome of Staphylococcus aureus from hospitals in South Western Nigeria. Indian Journal of Medical Microbiology, (2012): 30(2),159-64.
- Johnsson D, Molling P, Stralin K. Detection of Panton–Valentine leukocidin gene in Staphylococcus aureus by LightCycler PCR: clinical and epidemiological aspects. European Society of Clinical Microbiology and Infectious Diseases. 2004:10(10), 884-889.
- Finck-Barbancon V, Pre´vost G, Pie´mont Y. Improved purification of leukocidin from Staphylococcus aureus and toxin distribution among hospital strains. Res Microbiol 1991: 142, 75– 85.
- Lina G, Piemont Y, Godail-Gamot F et al. Involvement of Panton–Valentine leukocidin-producing Staphylococcus aureus in primary skin infections and pneumonia. Clin Infect Dis 1999: 29, 1128–1132.
- Couppie´ P, Criebier B, Pre´vost G, Grosshans E, Pie´mont Y. Leukocidin from Staphylococcus aureus and cutaneous infections: an epidemiologic study. Arch Dermatol 1994:130, 1208–1209.
- Pre´vost G, Couppie´ P, Pre´vost P et al. Epidemiological data on Staphylococcus aureus strains producing synergohymenotropic toxins. J Med Microbiol 1995: 42, 237–245.
- Antibacterial Activity of Ethanolic extract of Cinnamon against clinical Isolates of Staphylococcus aureus
Abstract Views :165 |
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Authors
Affiliations
1 Department of Microbiology, Saveetha Dental College and Hospital, Chennai, Tamilnadu, IN
2 Department of Orthodontics, Saveetha Dental College and Hospitals, Chennai, Tamil Nadu, IN
1 Department of Microbiology, Saveetha Dental College and Hospital, Chennai, Tamilnadu, IN
2 Department of Orthodontics, Saveetha Dental College and Hospitals, Chennai, Tamil Nadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 1 (2019), Pagination: 259-261Abstract
Staphylococci can cause many forms of infection like superficial skin lesions, osteomyelitis and endocarditis and more serious skin infections ,nosocomial infection of surgical wounds.They are Gram-positive cocci 1μm in diameter.The aim of our study was to determine the antibacterial activity of cinnamon bark oil against Grampositive isolates belonging to StaphylococcusKeywords
Staphylococcus Aureus, Cinnamon, Ethanol, Resistance.References
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- Linda S. M. Ooi et al, Antimicrobial Activities of Cinnamon Oil and Cinnamaldehyde from the Chinese Medicinal Herb Cinnamomum cassia Blume .Am. J. Chin. Med. 34, 511 (2006).
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- Role of Spices and Herbs in Controlling Dental Problems
Abstract Views :75 |
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Authors
Affiliations
1 Graduate Student, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai., IN
2 Assistant Professor, Department of Microbiology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai., IN
3 Tutor, Department of Public Health Dentistry, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai., IN
1 Graduate Student, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai., IN
2 Assistant Professor, Department of Microbiology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai., IN
3 Tutor, Department of Public Health Dentistry, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai., IN
Source
Research Journal of Pharmacology and Pharmacodynamics, Vol 14, No 1 (2022), Pagination: 23-28Abstract
Herbs and spices are not just useful to us as flavouring agents but they are actually nature's way to treat dental infections also. Several dental problems like tooth ache, caries, etc have become very common among the human population across the globe. These are mainly due to the action of microorganisms like Streptococcus mutans, Streptococcus sobrinus, lactobacilli, etc. Such problems can actually be cured naturally by using herbs and spices like cloves, thyme, peppermint leaves, goldenseal, green tea, etc which contain particular substances (that are found in dental medicines), which act against the microorganisms causing dental problems by either killing them or inhibiting their growth, are used to treat these problems. Apart from the above use, it is also effective in cases of infections where allopathic medications have no or minimal actions which may be due to the increased resistance exhibited by the pathogens to particular allopathic medications, some antibacterial agents currently used in dentistry produces adverse-affects like nausea, vomiting, diarrhoea, headache, staining of teeth, etc. and financial considerations in relation to allopathic medications in developing countries creates a need for alternative prevention and treatment options that are safe, effective and economical. Hence, the search for alternative products continues and herbs and spices that were used as traditional medicines in the ancient times are considered as good alternatives. Therefore, understanding the role of herbs and spices in controlling dental infection scan help people to cure and prevent these infections in a natural way and help them overcome all the above problemsKeywords
Spices, Herbs, Dental, Oral, Caries.References
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